I've heard of this issue before when a path to storage is lost. Do you have one path to storage, or more than one? Are you using software iSCSI or iSCSI hba's? If sw, make sure you have two pNICs allocated to the portgroup that you are using for iSCSI, and if hw iSCSI hba's, make sure both hba's (assuming you have two here) are configured for the LUNs.
-KjB
vExpert/VCP/VCAP
vmwise.com / @vmwise
-KjB